Av-600                   TopSpin 1.3 PL4

Start

     Start up Topspin

     Change Samples

     Standard Shim Settings

     Lock

     Browse to group directory

     Create new 1H filename

     Parameters

     Wobb

            ATMM & ATMA on ATM enabled probes

     Shim with BSMS keypad

Acquire 1H

     ii rga zg efp

     Exand & Phase

     Gradient Shimming

     Check LineShape

     Set Parameters

Processing

     Calibrate

     Baseline Correction

     Integrals

     Peak Picking

Plotting

     Plot Editor

     Export Plots as .emf .pdf

     Stack Plots from display window

Acquire Other Nuclei

     13C & DEPT-135

     31P

Finish

     Ready for next user

 Advanced

     Set SW & do Pulse Calibration

     HMQC-HMBC

            Process QC & BC with XwinNMR on SGI O2

     COSY    2D      Refer to HMQC-HMBC handout & NOESY handout for running Gradient COSY

     HomoNuclear-Decoupling    1D

     NOESY    2D

            Process NOESY with XwinNMR on SGI O2

            Process NOESY with TopSpin

     NOE Difference    1D

LCNMR Hystar 2.3                LCNMR data acquisition using TopSpin

Save data to DVD

Bruker Avance-600        XwinNMR           Instruction manuals

Setting up 1H and 13C ..............................31P & other X-nuclei

Transfer Data from AMX-600 SGI Indy computer to SGI O2 Workstation computer

XWINPLOT vers.3.1 ------1D plotting instructions

Advanced 1D plotting with XWINPLOT vers.3.1

Integration.......................Signal-to-Noise.......................T1..............................Presaturation

Homo-Nuclear-Decoupling...............................Paramagnetics..............................

Relaxation

COSY Acquisition ..                        {XwinNmr 3.5}                 .COSY Plottting (XWINPLOT)

HMQC / HMBC Acquisition...                  {XwinNmr 3.5}..                      ..QC/BC Plottting (XwinPlot)

Setting up TOCSY (presaturation)..............................

Setting up NOESY ..............................

NOE Difference..............................

LCNMR Hystar 2.3                LCNMR data acquisition using TopSpin

Tuning C13 probe..............................

EDTE                 {XwinNmr 3.5}.

Setting up Stack Plots..............................

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Copyright © 2005, The University of Iowa, High Field NMR Central Research Facility, Office of the Vice President for Research

Last modified: Thursday, 18-Nov-2005, J. Snyder

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